00178063

open access

Version of Record

ブドウ キュウキンガイ ドクソ ノ メンエキ カッセイ ニ カンスル ケンキュウ

Effect of Staphylococcal Exotoxins against T Cell Subsets in Spleen and Thymus of Mice

Japanese (日本語)

神戸大学農学部研究報告

22(1)

7-12

神戸大学農学部

1996-01-30

2006-09-15

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

Staphylococcal enterotoxins (SEs) and toxic shock syndrome toxin-one (TSST-1) produced by Staphylococcus aureus are responsible for a number of diseases with different symptoms. These toxins are known as so called 'super antigen'. Super antigens activate extraordinarily T cells to release excessive cytokines, such as tumor necrosis factor and interleukin-2,which is considered to be the mechanism of pathogenesis of the diseases. This study deals with flowcytometric analysis of T cell subsets in the spleen and thymus of mice that were inoculated intraperitoneally with purified staphylococcal exotoxins of SEA, SEB, SEC, SED and TSST-1. The results are summerized as follows. 1) In the thymus of a mouse inoculated with 25ng of TSST-1,both of the helper and cytotoxic T cells increased remarkably. In the spleen of the mouse both subsets of T cells slightly increased. The helper T cells in the thymus increased remarkably in a mouse inoculated with a small amount (17pg=1.7×(10)^<-2>ng) of TSST-1. 2) Decrease of the total cells of thymus and increase of the total cells of spleen were noted in mice inoculated with each 25ng of SEA and SEB. In mice inoculated with each 25ng of SEC and SED, increase of the immature T cells and the total cells of thymus was noted. In the thymus of mice inoculated with each 17pg of SEA-SED, the helper T cells increased, and the cytotoxic T cells also increased with exception of the case of SEC. This in vivo study clearly showed that with a small amount of staphylococcal exotoxins, especially TSST-1,the numbers of helper and cytotoxic T cells in the thymus and spleen are considerably affected.

紀要論文