00188180

open access

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A Computer Algorithm for the Standardization of Marker Size Obtained by Electrophoresis for Microsatellite Marker Analysis

English (英語)

Bulletin of allied medical sciences Kobe : BAMS (Kobe)

13

137-141

神戸大学医学部保健学科

1997-12-26

2006-09-12

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

We have applied the microsatellite marker anaysis to identify the gene loci that predispose to rheumatoid arthritis (RA). During execution of an improved computational algorithm after Holmans & Clayton, which is able to estimate the missing marker genotypes of parents by incorporating one unaffected sibling into an affected sib pair, we encountered the problem that the sizes of microsatellite markers obtained by electrophoresis were not always concentrated strictly around the expected, two-base distant, values. To overcome this problem, we have devised a new algorithm to standardize the size of marker alleles for automatic computational processing. Three feasible markers were identified under the criterion of the lod score being more than the prescribed level 3, which is generally used for the linkage analysis.

Rheumatoid arthritis

紀要論文