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コムギゾク ショクブツ ノ アミラーゼ アイソザイム ニ カンスル ケンキュウ 4 フツウケイ コムギ ノ アミラーゼ アイソザイム イデンシ ノ ザジョウ シテイル センショクタイ ノドウテイ ショクブツ ボウエキガク

STUDIES ON AMYLASE ISOZYMES OF TRITICEAE : IV. Chromosomal Identification of Genes Controlling Amylase Isozymes in Hexaploid Wheat

Japanese (日本語)

神戸大学農学部研究報告

14(2)

313-319

神戸大学農学部

1981-01-30

2006-09-15

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

The present study was carried out to identify the chromosome locating the stractual gene (s) for amylase isozymes in hexaploid wheat. For this purpose, an analysis of amylase isozymes was made on the aneuploids of Triticum aestivum cv. Chinese Spring, i. e. 9 lines of nullisomics, 27 lines of nulli-tetrasomics, 33 lines of ditelosomics and 7 lines of nulli-pentaploids (Table 1 and Fig. 1). The results obtained are summarized in the following. By the disc-isoelectrophocusing in acrylamide gel, 22 isozyme bands were able to be identified in the amylase zymogram from germinated seed of Chinese Spring disomics. They were classified into 3 groups. The first group was identified as α-amylase, the second as β-amylase, and the third as unidentified ones. The 3 nullisomics (nulli-4A, unlli-6A, nulli-6D), the 9 nulli-tetrasomics (nulli-5B tetra-5A, nulli-B tetra-5D, nulli-6D tetra-6A, nulli-6D tetra-6B, nulli-6A tetra-6B, nulli-7A tetra-7B, nulli-7A tetra-7D, nulli-7B tetra-7A, nulli-7B tetra-7D), the 7 ditelosomics (ditelo-4Aα, ditelo-6Aα, ditelo-7AS, ditelo-7BS, ditelo-3DS, ditelo-6Dα, ditelo-7DS) and the 3 nulli-pentaploids (nulli-penta 3D, nulli-penta 6D, nulli-penta 7D) were different from the disomic control in their isozyme patternes (Fig. 2,3 and 4). Thus, the chromosome armes involving gene (s) for amylase isozymes may be identified as follows : 5BL, 6Dβ, 6Aβ, and 4Aβ for α-3,α-5,α-6 and α-7 of α-amylase isozymes, respectively; 4Aβ and 7DL for β-1 and β-3 of β-amylase isozymes, respectively; 3DS for all of 3 isozymes, β-5,β-7 and β-9; and 7AL, 7BL, and 7DL for III-1 and III-3,III-4 and III-5,and III-2 of No. 3 zones amylase isozymes, respectively.

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