00228534

open access

Version of Record

ハンノウ ノ コトナル Nicotianaゾク ショクブツ ノ クキ ニ ドウニュウ サレタ タバコ モザイク ウイルス ノ ドウコウ 1 N tabacum cv Samsun ショクブツ ボウエキガク

The Behavior of Tobacco Mosaic Virus Introduced into the Stem of Some Nicotiana Plants Responding Differently to its Infection (I) : In the Plant of N. tabacum cv. Samsun

Japanese (日本語)

神戸大学農学部研究報告

12(2)

197-203

神戸大学農学部

1977

2006-09-15

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

Experiments have been conducted to elucidate the behavior of tobacco mosaic virus (TMV) within the plant of Nicotiana tabacum cv. Samsun, which responds usually with systemic mosaic mottle to TMV infection, when TMV is introduced into the stem through the cut stem-end. Throughout the experiments, all the plants of N. tabacum cv. Samsun tested were water-cultured individually after excising roots at the basal portion of the stem and were allowed to take up the aqueous suspension of purified TMV (ordinary strain) through the cut stem-end for some time, immediately after or considerably after removing roots. The experimental results obtained are summarized as follows : (1) Very few plants became infected when the virus was administered for various periods from 15 min. to 3hr., 24-120hr. after removing roots, but many plants became infected only when further treatment was added such as excising or rubbing the stem at the portion of 6-21cm above the stem-end after taking up TMV. Also, percentage infected was about 10% when TMV was taken up for periods of 15-60min. just after removing roots, whereas about 70% when further treatment of excising was added at the portion of 5-12cm above the stem-end after taking up TMV. (2) The virus was always detected from the portions of 6 or 10cm above the stem-end just after or 1-2 days after taking up TMV for periods from 30min. to 2hr., but not from the portions such as mentioned above 3 days or more after taking up TMV. (3) In a test, in which TMV was taken up through the upper or lower cut stem-end just after excising both the stem-ends of all tested plants, most plants tested became infected when TMV was taken up through lower one and additional excising was made at the portion of 10cm above the lower stem-end just after taking up TMV for 10min., whereas comparable results were obtained only when TMV was taken up through upper one and further excising was added at the portion of 10cm below the upper stem-end after taking up TMV for 2hr. (4) High percentage infected of about 100 was obtained when upper or lower cut stem-ends were inoculated with TMV by the dip-and-cut procedure in which each the stem-end was dipped into TMV suspension for 40 seconds and was excised immediately at the wet portion. The movement of TMV to the opposite direction from each the cut stem-end where inoculation was made was very slow and the rate of the movement was estimated to be cell-to-cell spread. In this case, moreover, it seemed that the virus movement of upward direction was always faster than that of downward direction.

紀要論文