00228535

open access

Version of Record

ハンノウ ノ コトナル Nicotianaゾク ショクブツ ノ クキ ニ ドウニュウ サレタ タバコ モザイク ウイルス ノ ドウコウ 2 N tabacum cv Samsun NN ショクブツ ボウエキガク

The Behavior of Tobacco Mosaic Virus Introduced into the Stem of Some Nicotiana Plants Responding Differently to its Infection (II) : In the Plant of N. tabacum cv. Samsun NN

Japanese (日本語)

神戸大学農学部研究報告

12(2)

205-209

神戸大学農学部

1977

2006-09-15

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

Experiments have been carried out to elucidate the behavior of tobacco mosaic virus (TMV) within the plant of Nicotiana tabacum cv. Samsun NN, which responds usually with local necrotic lesions to TMV infection, when TMV is introduced into the stem through the cut stem-end. Throughout the experiments, all the plants of N. tabacum cv. Samsun NN tested were water-cultured individually after excising roots at the basal portion of the stem and were allowed to take up the aqueous suspension of purified TMV (ordinary strain) through the cut stem-end for some time, immediately after or considerably after removing roots. Since it was shown that most plants tested did not become infected under the condition of usual temperatures (20-29℃) without exceptional ones showing sudden top necrosis, most of the results were obtained from the experiments made under the condition of 30℃ where the plants became infected systemically and developed mosaic mottle. The experimental results obtained are summarized as follows : (1) Very few plants became infected when TMV was administered for various periods from 30min. to 3hr., 72-120hr. after removing roots, but many plants (about 50%) became infected only when further treatment was added such as excising or rubbing the stem at the portion of 6-20cm above the stem-end after taking up TMV. Also, percentages infected were about 80,40 and 20% when further treatment of excising was added respectively at the portions of 5-7,10-12 and 21cm above the stem-end from which TMV was taken up for periods of 5-60min. just after removing roots. (2) In a test, in which TMV was taken up through the upper or lower cut stem-end just after excising both the stem-ends of all tested plants, most plants tested became infected when TMV was taken up through lower one and additional excising was made at the portion of 6-10cm above the lower stem-end just after taking up TMV for 10min., whereas comparable results were obtained only when TMV was taken up through upper one and further excising was added at the portion of 6-10cm below the upper stem-end after taking up TMV for 1hr. (3) These results suggest that TMV which was introduced into the stem of N. tabacum cv. Samsun NN through the cut stem-end in the manner of these experiments remains only in the xylem tissues without any tissues being infected even under the condition of 30℃, as well as in the case of N. tabacum cv. Samsun.

紀要論文