00228581

open access

Version of Record

カフンリュウ ノ ハイ ケイセイ キコウ ニカンスル ケンキュウ ダイ3ポウ コトナル ショトウ ノウド ニタイスル タバコ ヤク バイヨウ カフンリュウ ノ ブンレツ ハンノウセイ オヨビ ハイジョウタイ ケイセイ カテイ エンゲイ ノウガク

English (英語)

神戸大学農学部研究報告

13(2)

193-202

神戸大学農学部

1979

2006-09-15

本文ファイルは、国立情報学研究所CiNiiより提供されたものです。

Anthers of tobacco plants (Nicotiana tabacum L. cv. Bright Yellow, 2n=48) were cultured on agar media containing sucrose in varied concentrations to assess the effects of sucrose on the mitotic behaviors of pollen grains. Furthermore, a possible process of pollen embryogenesis was discussed on the basis of the data concerning mitotic behavior of embryogenic pollen grains and their occurrence frequency. The most favorable sucrose concentration was 1/4M not only for the induction of multinucleate and multicellular grains but for the formation of embryoids and plantlets. Moreover, sucrose was also effective in giving rise to the vegetative nuclear divisions in its high concentration. On the basis of the present results, the process of pollen embryogenesis was proposed as shown in Fig. 5 of text. Namely, two routes, A and B, are firstly considered in this case. The A route initiates from the G+V type-grains, followed by repeated divisions of the vegetative nuclei only. When the number of nuclei reached 5-8,further three types become distinguished in the pollen grains. One of them is a type that the visible cell wall formation with degeneration of generative nuclei has been induced (A-1 route), and the others are types that the pollen grains containing one or two generative nuclei continue to divide their vegetative nuclei till reaching about 15 nuclei without forming visible cell walls (A-2 and A-3 routes). The B route intiates from the 2V type-grains and subsequently leads to repeated divisions of the vegetative nuclei. Although every one of these 4 routes may be responsible for pollen embryogenesis, it seems probable to consider that the A route plays a more important role for pollen embryogenesis in tobacco anther culture.

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