Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef

Ueda, Shuji; Takashima, Yasuharu; Gotou, Yunosuke; Sasaki, Ryo; Nakabayashi, Rio; Suzuki, Takeshi; Sasazaki, Shinji; Fukuda, Ituko; Kebede, Biniam; Kadowaki, Yuki; Tamura, Maiko; Nakanishi, Hiroki; Shirai, Yasuhito

https://hdl.handle.net/20.500.14094/0100476812

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メタデータID	0100476812
アクセス権	open access
出版タイプ	Version of Record
タイトル	Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef
著者	Ueda, Shuji ; Takashima, Yasuharu ; Gotou, Yunosuke ; Sasaki, Ryo ; Nakabayashi, Rio ; Suzuki, Takeshi ; Sasazaki, Shinji ; Fukuda, Ituko ; Kebede, Biniam ; Kadowaki, Yuki ; Tamura, Maiko ; Nakanishi, Hiroki ; Shirai, Yasuhito
著者ID A1058 研究者ID 1000050379400 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=06b23bdf963d8d6a520e17560c007669 著者名 Ueda, Shuji 上田, 修司ウエダ, シュウジ所属機関名農学研究科
著者名 Takashima, Yasuharu
著者名 Gotou, Yunosuke
著者名 Sasaki, Ryo
著者名 Nakabayashi, Rio
著者ID A1040 研究者ID 1000010321952 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f10a7284930bd309520e17560c007669 著者名 Suzuki, Takeshi 鈴木, 武志スズキ, タケシ所属機関名農学研究科
著者ID A1055 研究者ID 1000050457115 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=2c817e1d7e37be6f520e17560c007669 著者名 Sasazaki, Shinji 笹崎, 晋史ササザキ, シンジ所属機関名農学研究科
著者名 Fukuda, Ituko
著者名 Kebede, Biniam
著者名 Kadowaki, Yuki
著者名 Tamura, Maiko
著者名 Nakanishi, Hiroki
著者ID A1196 研究者ID 1000060263399 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=3152854cdb3a4eae520e17560c007669 著者名 Shirai, Yasuhito 白井, 康仁シライ, ヤスヒト所属機関名農学研究科
言語	English (英語)
収録物名	Metabolites
巻(号)	12(9)
ページ	777
出版者	MDPI
刊行日	2022-09
公開日	2022-10-04
抄録	Japanese Black cattle (Japanese Wagyu) beef is attracting attention for its aroma and marbling, and its handling is increasing worldwide. Here, we focused on the origin discrimination of Wagyu beef and analyzed the nutritional components of Japanese Wagyu (produced in multiple prefectures of Japan), Hybrid Wagyu (a cross between Angus and Wagyu cattle born in Australia and transported to Japan), and Australian Wagyu beef using mass spectrometry (MS). Triple-quadrupole liquid chromatography–MS was used to clarify the molecular species of lipids in Wagyu beef. Fourteen classes of lipids were separated, and 128 different triacylglycerides (TGs) were detected. A simple comparative analysis of these TGs using high-performance liquid chromatography revealed significantly higher levels of triolein (C18:1/C18:1/C18:1; abbreviated OOO) and C18:1/C18:1/C16:1 (OOPo) in Japanese Wagyu. Wagyu elements beef were comprehensively analyzed using inductively coupled plasma (ICP)–MS and ICP–optical emission spectrometry. We found significant differences in the rubidium, cesium, and lithium levels of Japanese and Australian Wagyu beef. On comparing metabolites using gas chromatography–MS, we identified significant differences in the levels of amino acids and other components of the Japanese and Australian Wagyu beef. These results suggest the possibility of determining the origin of Wagyu cattle breeds using MS and genetic discrimination.
キーワード	Japanese Black cattle
	metabolomics
	origin determination
	Hybrid Wagyu
	triolein
カテゴリ	農学研究科
カテゴリ	学術雑誌論文
権利	© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
権利	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
関連情報	DOI https://doi.org/10.3390/metabo12090777

資源タイプ	journal article
eISSN	2218-1989　OPACで所蔵を検索　 CiNiiで学外所蔵を検索

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