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https://hdl.handle.net/20.500.14094/0100482157

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メタデータID
0100482157
アクセス権
open access
出版タイプ
Version of Record
タイトル
Growth and Migration Blocking Effect of Nanaomycin K, a Compound Produced by Streptomyces sp., on Prostate Cancer Cell Lines In Vitro and In Vivo
著者
Hirata, Yuto ; Shigemura, Katsumi ; Moriwaki, Michika ; Iwatsuki, Masato ; Kan, Yuki ; Ooya, Tooru ; Maeda, Koki ; Yang, Youngmin ; Nakashima, Takuji ; Matsuo, Hirotaka ; Nakanishi, Jun ; Fujisawa, Masato
著者名
Hirata, Yuto
著者ID
A1467
研究者ID
1000000457102
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=00a87f636663131d520e17560c007669
著者名
Shigemura, Katsumi
重村, 克巳
シゲムラ, カツミ
所属機関名
保健学研究科
著者名
Moriwaki, Michika
著者名
Iwatsuki, Masato
著者名
Kan, Yuki
著者ID
A0016
研究者ID
1000010301201
ORCID
0000-0001-7766-5455
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=22c572387224196f520e17560c007669
著者名
Ooya, Tooru
大谷, 亨
オオヤ, トオル
所属機関名
工学研究科
著者名
Maeda, Koki
著者名
Yang, Youngmin
著者名
Nakashima, Takuji
著者名
Matsuo, Hirotaka
著者名
Nakanishi, Jun
著者ID
A0878
研究者ID
1000030243314
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=57739d893d9102e6520e17560c007669
著者名
Fujisawa, Masato
藤澤, 正人
フジサワ, マサト
所属機関名
医学研究科
言語
English (英語)
収録物名
Cancers
巻(号)
15(10)
ページ
2684
出版者
MDPI
刊行日
2023-05
公開日
2023-06-12
抄録
Since castration-resistant prostate cancer (CRPC) acquires resistance to molecularly targeted drugs, discovering a class of drugs with different mechanisms of action is needed for more efficient treatment. In this study, we investigated the anti-tumor effects of nanaomycin K, derived from “Streptomyces rosa subsp. notoensis” OS-3966. The cell lines used were LNCaP (non-CRPC), PC-3 (CRPC), and TRAMP-C2 (CRPC). Experiments included cell proliferation analysis, wound healing analysis, and Western blotting. In addition, nanaomycin K was administered intratumorally to TRAMP-C2 carcinoma-bearing mice to assess effects on tumor growth. Furthermore, immuno-histochemistry staining was performed on excised tissues. Nanaomycin K suppressed cell proliferation in all cell lines (p < 0.001) and suppressed wound healing in TRAMP-C2 (p = 0.008). Nanaomycin K suppressed or showed a tendency to suppress the expression of N-cadherin, Vimentin, Slug, and Ras in all cell lines, and suppressed the phosphorylation of p38, SAPK/JNK, and Erk1/2 in LNCaP and TRAMP-C2. In vivo, nanaomycin K safely inhibited tumor growth (p = 0.001). In addition, suppression of phospho-Erk1/2 and increased expression of E-cadherin and cleaved-Caspase3 were observed in excised tumors. Nanaomycin K inhibits tumor growth and suppresses migration by inhibiting epithelial-mesenchymal transition in prostate cancer. Its mechanism of action is related to the inhibition of phosphorylation of the MAPK signaling pathway.
キーワード
nanaomycin K
streptomyces
prostate cancer
castration-resistant prostate cancer
tumor growth
migration
epithelial mesenchymal transition
MAPK signaling pathway
カテゴリ
医学研究科
工学研究科
保健学研究科
学術雑誌論文
権利
© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
CC license
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
関連情報
DOI
https://doi.org/10.3390/cancers15102684