0100482714

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Characterization of FLOWERING LOCUS C 5 in Brassica rapa L.

English (英語)

Molecular Breeding

43(3)

58

Springer Nature B.V.

2023-07-19

2023-07-31

Brassica rapa L., which includes Chinese cabbage, turnip, and pak choi, has more complex flowering time regulation than does Arabidopsis thaliana due to the presence of multiple paralogous flowering time genes. FLOWERING LOCUS C (FLC) is one of the key genes regulating the flowering time, and B. rapa has four FLC paralogs. BrFLC₅ on the reference genome is deemed a pseudogene because of a mutation (from G to A) in the splice site of the third intron, but there are some accessions with a G nucleotide in the splice site. In this study, we genotyped 310 B. rapa accessions and found that 19 had homozygous and 81 had heterozygous putative functional BrFLC₅ alleles. Accessions of turnip showed the highest proportion with a functional BrFLC₅ allele. BrFLC₅ acts as a floral repressor when overexpressed in A. thaliana. The BrFLC₅ expression level varied in pre-vernalized plants, and this transcriptional variation was not associated with the G/A polymorphism in the third intron. Three accessions having a higher BrFLC₅ expression in pre-vernalized plants had a 584-bp insertion in the promoter region. Many regions homologous to this 584-bp sequence are present in the B. rapa genome, and this 584-bp inserted region has tandem duplications of an AT-rich sequence in its central region. The possibility that a high expression of a functional BrFLC₅ could contribute to producing premature bolting-resistant lines in B. rapa vegetables is discussed.

Brassica

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