神戸大学附属図書館デジタルアーカイブ
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https://hdl.handle.net/20.500.14094/0100485978
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2024-06-30
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0100485978 (fulltext)
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0100485978_suppl (fulltext)
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ファイル出力
メタデータID
0100485978
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel
著者
Yoshida, Kohsuke ; Tani, Hiromi ; Nobuhara, Sae ; Yamamoto, Shinji
著者ID
A3106
研究者ID
1000080452499
ORCID
0000-0002-8728-5226
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=60cc3adb46a7925e520e17560c007669
著者名
Yoshida, Kohsuke
吉田, 幸祐
ヨシダ, コウスケ
所属機関名
保健学研究科
著者名
Tani, Hiromi
著者名
Nobuhara, Sae
著者名
Yamamoto, Shinji
収録物名
Biopreservation and Biobanking
出版者
Mary Ann Liebert
刊行日
2023-11-09
公開日
2023-12-27
抄録
Objectives: The aim of this study was to determine the cause of elevated serum potassium levels when blood collection tubes containing separating gel are stored under refrigeration. Methods: Fifty-seven hospitalized patients and 11 healthy volunteers were recruited. Venous blood samples were obtained using Insepac II, Neotube, and Venoject® II, without anticoagulant. After centrifugation under different processing conditions, the capped tubes were stored at 4°C without aliquoting, and serum potassium levels were measured for up to 14 days. Correlation between the increase in potassium levels and blood cell counts was assessed. Furthermore, serum was replaced with a saline solution and potassium levels were determined after refrigeration. Results: Refrigerated samples stored in Insepac II tubes had significantly higher serum potassium levels on day 14 than on the day of blood collection. The increase in serum potassium levels was positively correlated with the number of red blood cells, but not white blood cells and platelets in venous blood. Furthermore, potassium levels were elevated when serum was replaced with a saline solution. Using Venoject II, which has a larger tube diameter and thicker separating gel than those of Insepac II and Neotube, did not increase serum potassium levels after storage. Increase in the serum potassium level was markedly suppressed by centrifugation at 2330 g for 15 minutes relative to other processing conditions. Conclusions: Potassium levels increase when serum is refrigerated in collection tubes containing separating gel. This can be attributed to contamination of the serum layer by blood cell components beyond the separating gel.
キーワード
refrigeration
blood collection
serum potassium
blood clots
haemolysis
カテゴリ
保健学研究科
学術雑誌論文
権利
This is the accepted version of the following article: [Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel. Kohsuke Yoshida, Hiromi Tani, Sae Nobuhara, and Shinji Yamamoto. Biopreservation and Biobanking], which has now been formally published in final form at Biopreservation and Biobanking at https://doi.org/10.1089/bio.2022.0217. This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions.
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資源タイプ
journal article
言語
English (英語)
ISSN
1947-5535
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eISSN
1947-5543
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関連情報
DOI
https://doi.org/10.1089/bio.2022.0217
PMID
37943629
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