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A NEW ENZYMATIC METHOD FOR THE DETERMINATION OF SIALIC ACID IN SERUM AND ITS APPLICATION FOR A MARKER OF ACUTE PHASE REACTANTS

English (英語)

The Kobe journal of the medical sciences

27(3)

91-102

1981-06

An enzymatic method for the measurement of sialic acid in serum is described. Neuraminidase [sialidase, acylneuraminyl hydrolase, EC 3.2. 1.18] from Arthrobacter ureafaciens is used for the enzymatic hydrolysis of sialyl compounds. In the presence of N-acetyl neuraminic acid (NANA) aldolase [N-acetyl neuraminate pyruvate-lyase, EC 4.1.3.3], extracted from E. coli, free sialic acid is cleaved to N-acetyl-D-mannosamine and pyruvate, then pyruvate is reduced to lactate by coexisting lactic acid dehydrogenase (LDH) and NADH. A fixed time (two point) measurement is made by measuring the delta absorbance change in 15 min. Sensitivity and precision were excellent and linearity extends to 200 mg/dl of sialic acid concentration. The mean value of serum sialic acid of normal adults was 37 .8-64.2 mg/dl with no difference between males and females. Good correlation between sialic acid concentration and α1 -acid glycoprotein or α1 -antitrypsin was observed. Serum sialic acid concentration of the patients with acute myocardial infarction had kept high levels after the attack with no relation to released enzymes. A positive correlation was found for acute phase proteins and sialic acid. The measurement of sialic acid in serum seems to provide a rapid and simple index as acute phase reactants.

myocardial infarction

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