0100489026

open access

Version of Record

ESTABLISHMENT OF CELL LINE FROM CULEX MOSQUITO

English (英語)

The Kobe journal of the medical sciences

16(1)

41-50

1970-03

The cell line established from the mosquito, Culex molestus, growing well in the medium supplemented with 10 volume percent of calf serum instead of the derivation of insect fluid, has been still continuing its growth for over 87th passage at 28°C in the author's laboratory. Population doubling time of this cell line was about 30 hr and chromosomes observed in 80th passage were 2n(=6) in the main. For the convenience of arboviruses investigations, a modification of the culture medium was made and the following results were preliminary obtained; those media supplemented with one volume percent of either bovine plasma albumin or calf serum were able to maintain the cultures for over a month without showing any changes in their appearance, suggesting its promising availability for the virological research. The search for the cell line to be subcultured in the medium without calf serum is under investigation.

culture medium

紀要論文