0100489086

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PROPAGATION AND CYTOPATHIC EFFECTS OF JAPANESE ENCEPHALITIS AND RELATED VIRUSES IN MAMMALIAN CEREBELLAR TISSUE CULTURES

English (英語)

The Kobe journal of the medical sciences

13(1)

1-21

1967-03

Cerebellar tissues from puppies, kittens and human embryos were cultivated on a cover slip by the roller tube technique, using a culture medium consisting of human cancerous ascitic fluid, chick embryo extract and Gey's balanced salt solution with a high concentration of glucose. In any of the tissue cultures employed, three strains of mouse-passaged Japanese encephalitis (JE) virus and a strain of Negishi virus, a member of the Russian Spring-Summer encephalitis group, multiplied unequivocally. The highest virus titers in fluid phase were usually obtained 3 to 7 days after inoculation of virus. The grades of multiplication, however, were different from one virus-culture combination to another; for instance, growth of J ATH strain JE virus in any of the culture systems was significantly superior to that of K, I. strain JE virus. Under the conditions studied. viral growth in kitten cerebellar tissue cultures was generally lower than that in puppy or human embryo cerebellar cultures. Differences in the nervous tissue culture affinity among different virus strains were evident. In the virus-infected cultures, the Purkinje cells exhibited definite degeneration. The nuclei, cell bodies, dendrites and neurites, as well as neurofibrils showed characteristic changes revealed clearly by Bodian's sliver impregnation and/or Nissl stain. The alterations proceeded in the course of infection, and the cells were eventually disintegrated. The cytopathic effects were prevented by mixing the viruses with specific antiviral antisera prior to inoculation. In control cultures incubated under disadvantageous conditions such as prolonged incubation without changing medium or incubation at higher temperatures, the Purkinje cells showed certain abnormal appearances, but such alterations were distinguishable from those occurring inevitably in the vii-us-infected cultures. Virologic and neuropathologic significances of the findings are discussed.

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