0100489121

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RESEARCH ON DENGUE IN TISSUE CULTURE : II. Further Observations on Virus-Tissue Culture Affinity

English (英語)

The Kobe journal of the medical sciences

12(4)

189-198

1966

Attempts were made to have dengue virus adapt to cultures of trypsinized chick embryo skin-muscle cells. Multiplication of the mouse-passaged type 1 Mochizuki strain dengue virus in this culture system was equivocal, showing only a prolonged maintenance of active virus. When the virus was successfully passed through fuscatus monkey kidney cell cultures, it proved to multiply significantly better in the chick embryo cultures, compared with the original mouse-passaged virus. In either case, however, serial transmission through the cell cultures was unsuccessful. Both monkey kidney cell culture-passaged and mouse-passaged viruses of the Mochizuki strain multiplied well, exhibiting CPE, in cultures of an established African green monkey kidney cell line. In this system, "virus carrier state " or "persistent infection" took place. It was observed that the cellular degeneration was never total but that destruction and restoration of cellular sheets occurred intermittently and active virus was continually detected in the culture fluid for more than 400 days.

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