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https://hdl.handle.net/20.500.14094/0100490288

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メタデータID
0100490288
アクセス権
open access
出版タイプ
Version of Record
タイトル
The Improved Antigen Uptake and Presentation of Dendritic Cells Using Cell-Penetrating D-octaarginine-Linked PNVA-co-AA as a Novel Dendritic Cell-Based Vaccine
著者
Fujioka, Yuri ; Ueki, Hideto ; A, Ruhan ; Sasajima, Akari ; Tomono, Takumi ; Ukawa, Masami ; Yagi, Haruya ; Sakuma, Shinji ; Kitagawa, Koichi ; Shirakawa, Toshiro
著者名
Fujioka, Yuri
著者名
Ueki, Hideto
著者名
A, Ruhan
著者名
Sasajima, Akari
著者名
Tomono, Takumi
著者名
Ukawa, Masami
著者名
Yagi, Haruya
著者名
Sakuma, Shinji
著者ID
A2336
研究者ID
1000000822884
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail.html?systemId=6dbda5319243ba9b520e17560c007669
著者名
Kitagawa, Koichi
北川, 孝一
キタガワ, コウイチ
所属機関名
科学技術イノベーション研究科
著者ID
A0776
研究者ID
1000070335446
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail.html?systemId=e358a376badb297f520e17560c007669
著者名
Shirakawa, Toshiro
白川, 利朗
シラカワ, トシロウ
所属機関名
科学技術イノベーション研究科
言語
English (英語)
収録物名
International Journal of Molecular Sciences
巻(号)
25(11)
ページ
5997
出版者
MDPI
刊行日
2024-06
公開日
2024-06-28
抄録
Cancer immunotherapy using antigen-pulsed dendritic cells can induce strong cellular immune responses by priming cytotoxic T lymphocytes. In this study, we pulsed tumor cell lysates with VP-R8, a cell-penetrating D-octaarginine-linked co-polymer of N-vinylacetamide and acrylic acid (PNVA-co-AA), into the DC2.4 murine dendritic cell line to improve antigen uptake and then determined the anti-tumor effect in tumor-bearing mice. DC2.4 cells were pulsed with the cell lysate of EL4, a murine lymphoma cell line, and VP-R8 to generate the DC2.4 vaccine. For the in vivo study, DC2.4 cells pulsed with EL4 lysate and VP-R8 were subcutaneously injected into the inguinal lymph node to investigate the anti-tumor effect against EL4 and EL4-specific T cell immune responses. VP-R8 significantly improved antigen uptake into DC2.4 compared to conventional keyhole limpet hemocyanin (p < 0.05). The expression of MHC class I, MHC class II, and CD86 in DC2.4 cells significantly increased after pulsing tumor lysates with VP-R8 compared to other treatments (p < 0.05). The intra-lymph node injection of DC2.4 pulsed with both VP-R8 and EL4 lysate significantly decreased tumor growth compared to DC2.4 pulsed with KLH and lysates (p < 0.05) and induced tumor-infiltrating CD8T cells. The DC2.4 vaccine also remarkably increased the population of IFN-gamma-producing T cells and CTL activity against EL4 cells. In conclusion, we demonstrated that VP-R8 markedly enhances the efficiency of dendritic cell-based vaccines in priming robust anti-tumor immunity, suggesting its potential as a beneficial additive for dendritic cell-based immunotherapy.
キーワード
cancer immunotherapy
dendritic cell vaccine
cell-penetrating polymer
drug delivery system
カテゴリ
科学技術イノベーション研究科
学術雑誌論文
権利
© 2024 by the authors. Licensee MDPI, Basel, Switzerland.
CC license
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
関連情報
DOI
https://doi.org/10.3390/ijms25115997