0100491519

open access

Accepted Manuscript

Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica

English (英語)

Biotechnology Progress

40(6)

e3499

John Wiley & Sons

2024-11

2025-07-26

Published Online: 2024-07-26

Short-chain esters, particularly isobutyl acetate and isoamyl acetate, hold significant industrial value due to their wide-ranging applications in flavors, fragrances, solvents, and biofuels. In this study, we demonstrated the biosynthesis of acetate esters using Yarrowia lipolytica as a host by feeding alcohols to the yeast culture. Initially, we screened for optimal alcohol acyltransferases for ester biosynthesis in Y. lipolytica. Strains of Y. lipolytica expressing atf1 from Saccharomyces cerevisiae, produced 251 or 613 mg/L of isobutyl acetate or of isoamyl acetate, respectively. We found that introducing additional copies of ATF1 enhanced ester production. Furthermore, by increasing the supply of acetyl-CoA and refining the culture conditions, we achieved high production of isoamyl acetate, reaching titers of 3404 mg/L. We expanded our study to include the synthesis of a range of acetate esters, facilitated by enriching the culture medium with various alcohols. This study underscores the versatility and potential of Y. lipolytica in the industrial production of acetate esters.

Yarrowia lipolytica

学術雑誌論文

This is the peer reviewed version of the following article: [Koshiba A, Nakano M, Hirata Y, et al. Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica. Biotechnol. Prog. 2024; 40(6):e3499.], which has been published in final form at [https://doi.org/10.1002/btpr.3499]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.