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Amino acid deaminase outperforms transaminase in α-keto acid production from amino acids

English (英語)

Metabolic Engineering

96

234-242

Elsevier

2026-07

2026-04-10

α-Keto acids are central metabolic intermediates that serve as precursors for a broad range of valuable compounds, including alcohols, organic acids, and alkanes. Therefore, efficient biosynthesis of α-keto acids from α-amino acids is essential for microbial production of α-keto acids-derived products. While transaminases have traditionally been employed for this conversion, their dependence on α-ketoglutarate and reversibility can impose metabolic burdens and limit yield. Recently, amino acid deaminases (AADs) have been used for conversion of amino acids to α-keto acids, because AADs catalyze irreversible oxidative deamination without any co-substrate and thus may offer advantages for α-keto acid production. Here, we experimentally demonstrated the superiority of AADs over transaminases in amino acid-to-α-keto acid conversion for the first time, using the production of indole-3-acetic acid from tryptophan as a case study. Escherichia coli cells expressing AAD genes from Proteus myxofaciens or P. mirabilis exhibited higher production of indole-3-acetic acid and indole-3-pyruvic acid, which is a direct product from tryptophan and an intermediate for indole-3-acetic acid production, than transaminases. Our kinetic analysis revealed that AADs exhibited higher affinity towards tryptophan than transaminases, contributing to higher production rate. This is the first report to demonstrate the superiority of AADs over transaminases for α-keto acids production. Furthermore, AADs showed better performance in the bioconversion of leucine, valine, and phenylalanine to their corresponding α-keto acids than transaminases. These findings establish AADs as promising biocatalysts for α-keto acid synthesis, supporting their use in pathway design for bioproduction of α-keto acid-derived compounds.

Microbial production

学術雑誌論文

This is an open access article under the Creative Commons Attribution 4.0 International license