80070153

open access

Version of Record

ジアエンソサン ナトリウム ニヨル カクサン ノ ブンカイ - PCR ハンノウジ ノ コンタミネーション ジョキョ エノ リヨウ

Degradation of DNA by Sodium Hypochlorite

Japanese (日本語)

神戸大学医療技術短期大学部紀要

7

17-22

神戸大学医療技術短期大学部

1991

2007-11-21

Polymerase chain reaction (PCR) was widely applied on the gene-amplification in molecular biological technique. However, a false positive reaction by the contaminant DNA from exogenous sources in PCR was noticed. PCR-amplified DNA was the major sources of the contamination in PCR. In this paper, sodium hypochlorite was used to remove the contamination. Plasmid DNA from enteropathogenic Escherichia coli (ETEC) as a typical DNA was selected. Plasmid DNA and PCR-amplified DNA from ETEC were treated for several minutes with 1.0, 0.1, and 0.01% sodium hypochlorite, and purified by ethanol-precipitation method. All of the recovered DNAs were not amplified by PCR with the exception of plasmid DNA which was treated with 0.01% Sodium hypochlorite. These results suggest that the contaminant DNA can be reduced by the treatment with the suitable concentration of sodium hypochlorite.

紀要論文