80070242

open access

Version of Record

オンド コウバイ ゲル デンキ エイドウホウ TGGE ニオケル エイドウ ジョウケン ノ ケントウ

Studies on Conditions in Temperature Gradient Gel Electrophoresis

Japanese (日本語)

神戸大学医療技術短期大学部紀要

9

119-124

神戸大学医療技術短期大学部

1993

2007-11-21

To obtain constant data in experiments of Temperature Gradient Gel Electrophoresis (TGGE), attention to the dryness of gel and sample-loading on electrophoresis was paid strictly. And then, the electrophoresis was performed with 6 or 8% acrylamidegel slab (130×160×1mm) consisting of 20mM MOPS buffer (pH8.0), 2 % glycerol and 8M urea. By these im¬provements, the electrophoretic patterns of TGGE were clearer and reproducible. PCR-products without the replacement to the annealing buffer could be used directly. The synthetic oligonucle¬otide primer was purified by the high pressure liquid chromatography equipped with TSKge1 Oli¬go-DNA RP column (4.6×150mm). When each purified primer-set was applied to TGGE, their electrophoretic patterns were clearer.

紀要論文