81003784

open access

Version of Record

A New in vitro Invasion Model for Oral Cancer Using an Acellular Allogenic Dermal Matrix (Alloderm) : The Relationship among in vitro Invasion Activity, in vivo Invasion and Metastasis

English (英語)

The Kobe journal of the medical sciences

57(4)

128-136

Kobe University School of Medicine

2011

2012-03-19

The purpose of this study was to develop a new in vitro model of cancer invasion using a human dermal equivalent, AlloDerm®. A squamous cell carcinoma line (HSC-2, HSC-3 and OSC20) and a malignant melanoma line (G361) were cultured on AlloDerm® and evaluated histologically to examine destruction of the basement membrane and invasion into the dermis of AlloDerm®. HSC-3 and G361 cells invaded through the basement membrane of AlloDerm® at 21-28 days after seeding, while HSC-2 and OSC20 cells did not. Next, these cells were transplanted into the tongues of nude mice, and the degree of local invasiveness was examined histologically. HSC-3 and G361 cells invaded diffusely to the surrounding tongue tissue, although HSC-2 and OSC20 showed only expansive growth. Further, these cells were transplanted subcutaneously in nude mice to study metastatic activity. Regional and distant metastases were rarely observed after transplantation of HSC-2 and OSC20 cells. On the other hand, HSC-3 and G361 cells frequently metastasized. These findings show a close relationship among in vitro invasiveness on AlloDerm®, in vivo invasiveness, and metastatic activity. This experimental model using AlloDerm® is a potentially new in vitro model of cancer invasion.

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