神戸大学附属図書館デジタルアーカイブ
https://doi.org/10.24546/81012029

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メタデータID
81012029
アクセス権
open access
出版タイプ
Version of Record
タイトル
Vitamin D Ameliorates Kidney Ischemia Reperfusion Injury via Reduction of Inflammation and Myofibroblast Expansion
著者
Arfian, Nur ; Budiharjo, Santosa ; Dian Prasetyo Wibisono ; Wiwit Ananda Wahyu Setyaningsih ; Romi, Muhammad Mansyur ; Ramadhea Laila Afifa An-Nur Willya Saputri ; Edreana Khusnur Rofiah ; Trita Rahmanti ; Agustin, Maulidina ; Sari, Dwi Cahyani Ratna
著者名
Arfian, Nur
著者名
Budiharjo, Santosa
著者名
Dian Prasetyo Wibisono
著者名
Wiwit Ananda Wahyu Setyaningsih
著者名
Romi, Muhammad Mansyur
著者名
Ramadhea Laila Afifa An-Nur Willya Saputri
著者名
Edreana Khusnur Rofiah
著者名
Trita Rahmanti
著者名
Agustin, Maulidina
著者名
Sari, Dwi Cahyani Ratna
言語
English (英語)
収録物名
The Kobe journal of the medical sciences
巻(号)
65(4)
ページ
138-143
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2019
公開日
2020-03-30
抄録
The incidence rate of Acute Kidney Injury (AKI) gets escalated each year. Kidney ischemia/reperfusion injury (IR injury) is the main cause of AKI after major cardiovascular surgery, trauma, or kidney transplantation. Reperfusion is considered essential for ischemic tissue. However, the evidence revealed that reperfusion itself has impact in cellular destruction. Vitamin D is not only known as calcium regulating hormone, but also as renoprotective agent. This study aimed to investigate the effect of vitamin D treatment on kidney IR injury in mice. Kidney IR injury was performed using 30 minutes of bilateral clamping of renal pedicles, then released in male Swiss Webster mice (3 months, 30-40 grams, n=20), which were divided into three groups: sham operation (SO) group, IR injury (IRI) group, and IR injury with 0.25 μg/ kg body weight of vitamin D treatment (IR7+VD). Mice were terminated at day 7 post operation, kidneys were harvested and used for paraffin making, immunostaining and RNA extraction. Tubular injury was quantified based on Periodic Acid-Schiff’s (PAS) staining. Immunostaining was done for quantification of macrophage (CD68) and myofibroblast (α-SMA). Reverse Transcriptase PCR (RT-PCR) was done to examine Monocyte Chemoattractant Protein-1 (MCP-1) and Toll-like Receptor 4 (TLR4) mRNA expression. Kidney IR injury induced significant increase of tubular injury, which was associated with higher myofibroblast and macrophage number. Meanwhile, Vitamin D treatment significantly reduced tubular, myofibroblast and macrophage number. RTPCR revealed reduction of TLR4 and MCP-1 mRNA expressions after Vitamin D treatment (p<0.05 vs IR group). Vitamin D ameliorates kidney IR injury through reducing inflammation and myofibroblast formation.
カテゴリ
The Kobe journal of the medical sciences ＞ 65巻 ＞ 65巻4号（2019）
紀要論文
関連情報
URI
http://www.med.kobe-u.ac.jp/journal/contents.html