神戸大学附属図書館デジタルアーカイブ
https://doi.org/10.24546/81012031

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メタデータID
81012031
アクセス権
open access
出版タイプ
Version of Record
タイトル
Calcitriol Ameliorates Kidney Injury Through Reducing Podocytopathy, Tubular Injury, Inflammation and Fibrosis in 5/6 Subtotal Nephrectomy Model in Rats
著者
Sari, Dwi Cahyani Ratna ; Maulida Wijaya Putri ; Tiara Putri Leksono ; Chairunnisa, Nogati ; Gerry Nathan Reynaldi ; Benhard Christopher Simanjuntak ; Debora, Josephine ; Yunus, Junaedy ; Arfian, Nur
著者名
Sari, Dwi Cahyani Ratna
著者名
Maulida Wijaya Putri
著者名
Tiara Putri Leksono
著者名
Chairunnisa, Nogati
著者名
Gerry Nathan Reynaldi
著者名
Benhard Christopher Simanjuntak
著者名
Debora, Josephine
著者名
Yunus, Junaedy
著者名
Arfian, Nur
言語
English (英語)
収録物名
The Kobe journal of the medical sciences
巻(号)
65(5)
ページ
153-163
出版者
神戸大学医学部
Kobe University School of Medicine
刊行日
2019
公開日
2020-03-30
抄録
Chronic kidney diseases (CKDs) lead to end-stage renal diseases (ESRD) which are characterized by glomerulosclerosis, tubular injury, anemia, inflammation, and interstitial fibrosis. Vitamin D is known to have renal protective effects. However, its effects relate to low and high doses of Vitamin D in CKD model is still unknown. CKD was performed using 5/6 subtotal nephrectomy procedure in male Sprague Dawley rats (3 months old, 200-300 grams, SN group; n=6), then rats were sacrificed on day 14 after operation. Sham operation was used for control (SO group; n=6). Calcitriol was administered in two doses : 0.01 μg/mL/100 gramsBW/day (SND1 group; n=6) and 0.05 μg/mL/100 gramsBW/day (SND2 group; n=6) intraperitoneally for 14 days. Glomerulosclerosis and tubular injury score were examined using PAS staining, meanwhile, interstitial fibrosis area fraction was assessed with Sirius Red staining. RT-PCR was performed for assessing nephrin, podocin, IL-6, CD68, Collagen-1, and TGF-β1 mRNA expressions. Immunostaining (IHC) was carried out to observe macrophage (CD68) and myofibroblast (α-SMA). SN demonstrated CKD condition with higher tubular injury, glomerulosclerosis, interstitial fibrosis, and inflammation compared to SO. Calcitriol-treated group (especially SND2) demonstrated significant lower tubular injury, glomerulosclerosis, and interstitial fibrosis compared to SN. SND2 group showed not only significantly lower CD68, IL-6, Collagen-1, and TGF-β1 mRNA expressions, but also higher mRNA expressions of nephrin and podocin. SND2 group also demonstrated reduction of macrophages infiltration and myofibroblasts expansion based on its histopathological appearance. Vitamin D may have a renoprotective effect on 5/6 subtotal nephrectomy model by attenuating podocytopathy, tubular injury, inflammation and interstitial fibrosis.
カテゴリ
The Kobe journal of the medical sciences ＞ 65巻 ＞ 65巻5号（2019）
紀要論文
関連情報
URI
http://www.med.kobe-u.ac.jp/journal/contents.html