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https://hdl.handle.net/20.500.14094/90004542

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メタデータID
90004542
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Altered expression of SDF-1 and CXCR4 during fracture healing in diabetes mellitus
著者
Arakura, Michio ; Lee, Sang Yang ; Takahara, Shunsuke ; Okumachi, Etsuko ; Iwakura, Takashi ; Fukui, Tomoaki ; Nishida, Kotaro ; Kurosaka, Masahiro ; Kuroda, Ryosuke ; Niikura, Takahiro
著者名
Arakura, Michio
著者ID
A1448
研究者ID
1000040533732
著者名
Lee, Sang Yang
李, 相亮
リ, ソウリョウ
所属機関名
医学部附属病院
著者名
Takahara, Shunsuke
著者名
Okumachi, Etsuko
著者名
Iwakura, Takashi
著者名
Fukui, Tomoaki
著者ID
A1371
研究者ID
1000000379372
著者名
Nishida, Kotaro
西田, 康太郎
ニシダ, コウタロウ
所属機関名
医学研究科
著者名
Kurosaka, Masahiro
著者ID
A0783
研究者ID
1000080379362
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=f51cbfc82378ae63520e17560c007669
著者名
Kuroda, Ryosuke
黒田, 良祐
クロダ, リョウスケ
所属機関名
医学研究科
著者ID
A1418
研究者ID
1000040448171
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=9a23c5d02379d159520e17560c007669
著者名
Niikura, Takahiro
新倉, 隆宏
ニイクラ, タカヒロ
所属機関名
医学研究科
言語
English (英語)
収録物名
International Orthopaedics
巻(号)
41(6)
ページ
1211-1217
出版者
Springer
刊行日
2017-06
公開日
2018-07-01
抄録
Purpose Diabetes mellitus (DM) is known to impair fracture healing. The purpose of this study was to elucidate and compare the gene expression patterns and localization of stromal cell-derived factor 1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) during fracture healing of the femur in rats with and without DM. Methods Closed transverse fractures were created in the femurs of rats equally divided into a DM group and control group; DM was induced by streptozotocin. At post-fracture days five, seven, 11, 14, 21 and 28, total RNA was extracted from the fracture callus and mRNA expression levels of SDF-1 and CXCR4 were measured by real-time polymerase chain reaction. Localization of SDF-1 and CXCR4 proteins at the fracture site was determined by immunohistochemistry at days 21 and 28. Results SDF-1 expression was significantly lower in the DM group than in the healthy group on days 21 and 28, and showed a significant difference between days 14 and 21 in the healthy group. There was no significant difference in CXCR4 expression levels between the healthy and DM groups at any time point. On day 21 immunoreactivity of SDF-1 and CXCR4 was detected at the fracture site of the healthy group but no immunoreactivity was observed in the DM group. On day 28, immunoreactivity of SDF-1 and CXCR4 was detected at the fracture site in both groups. Conclusion Gene expression and localization of SDF-1 and CXCR4 was altered during fracture healing, which may contribute to the impaired fracture healing in DM.
キーワード
Diabetes mellitus
Fracture healing
Delayed union
Endochondral ossification
Stromal cell-derived factor 1
CXC chemokine receptor 4
カテゴリ
医学研究科
医学部附属病院
学術雑誌論文
権利
© SICOT aisbl 2017 This is a post-peer-review, pre-copyedit version of an article published in International Orthopaedics. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00264-017-3472-8
関連情報
DOI
https://doi.org/10.1007/s00264-017-3472-8