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https://hdl.handle.net/20.500.14094/90004867

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メタデータID
90004867
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Equine-like G3 rotavirus strains as predominant strains among children in Indonesia in 2015–2016
著者
Utsumi, Takako ; Wahyuni, Rury Mega ; Doan, Yen Hai ; Dinana, Zayyin ; Soegijanto, Soegeng ; Fujii, Yoshiki ; Juniastuti ; Yamani, Laura Navika ; Matsui, Chieko ; Deng, Lin ; Abe, Takayuki ; Soetjipto ; Lusida, Maria Inge ; Ishii, Koji ; Shimizu, Hiroyuki ; Katayama, Kazuhiko ; Shoji, Ikuo
著者ID
A1397
研究者ID
1000010364051
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=014a08eb3a44a329520e17560c007669
著者名
Utsumi, Takako
所属機関名
医学研究科
著者名
Wahyuni, Rury Mega
著者名
Doan, Yen Hai
著者名
Dinana, Zayyin
著者名
Soegijanto, Soegeng
著者名
Fujii, Yoshiki
著者名
Juniastuti
著者名
Yamani, Laura Navika
著者ID
A2224
研究者ID
1000070778414
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=a7a5713302f41316520e17560c007669
著者名
Matsui, Chieko
所属機関名
医学研究科
著者名
Deng, Lin
著者ID
A1961
研究者ID
1000090403203
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=e6d4911a059f186f520e17560c007669
著者名
Abe, Takayuki
所属機関名
医学研究科
著者名
Soetjipto
著者名
Lusida, Maria Inge
著者名
Ishii, Koji
著者名
Shimizu, Hiroyuki
著者名
Katayama, Kazuhiko
著者ID
A0795
研究者ID
1000040356241
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=72a067e0cb19ce45520e17560c007669
著者名
Shoji, Ikuo
所属機関名
医学研究科
言語
English (英語)
収録物名
Infection, Genetics and Evolution
巻(号)
61
ページ
224-228
出版者
Elsevier
刊行日
2018-07
公開日
2019-08-01
抄録
Rotavirus A (RVA) is a major cause of acute gastroenteritis in humans and animals worldwide. As a result of the segmented nature of the rotavirus genome, genetic reassortment commonly occurs. This study aims to clarify the genetic characteristics of RVAs circulating in Indonesia. From June 2015 through August 2016, stool samples were collected from 134 children aged <5 years (71 male and 63 female) with acute gastroenteritis who were inpatients at a private hospital in Surabaya, Indonesia. All stool samples were screened for RVA antigen using immunochromatography. Forty-two samples (31.3%, 42/134) were RVA antigen-positive. All RVA positive samples tested showed the unusual combinations of G3P[8] (n = 36) and G3P[6] (n = 3) with a short RNA pattern by G/P typing and polyacrylamide gel electrophoresis (PAGE). Whole genome analysis by next-generation sequencing (NGS) was performed for 11 strains to determine the RVA genotypes. Eleven rotavirus strains were found to carry a DS-like genetic backbone; nine strains showed a G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genome constellation, which was recently reported in Australia, Hungary, Spain and Brazil; as well, two strains showed a G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genome constellation. The phylogenetic tree based on the VP7 gene showed that all 11 strains were classified as equine-like G3, which is genetically distinct and different in origin from typical human G3 strains. The phylogenetic tree based on the NSP4 gene showed that six strains were classified as bovine-like strain and the remaining five were classified as human strain. In conclusion, we identified the strains which are intergenogroup reassortants containing an equine-like G3 VP7, a P[8])/P[6] VP4, with a DS-1-like genetic backbone. These findings suggest that equine-like G3P[8] and P[6] RVA strains have been circulating in the Indonesian population for at least 1 year and probably longer, indicating a diversity of RVAs in this area.
キーワード
Rotavirus
RVA
Gastroenteritis
Equine
Indonesia
Genetic reassortment
カテゴリ
医学研究科
学術雑誌論文
権利
© 2018 Elsevier B.V. All rights reserved.
CC license
This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
関連情報
DOI
https://doi.org/10.1016/j.meegid.2018.03.027
URI
http://www.kobe-u.ac.jp/research_at_kobe/NEWS/news/2018_05_22_01.html