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https://hdl.handle.net/20.500.14094/90004921

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メタデータID
90004921
アクセス権
open access
出版タイプ
Version of Record
タイトル
Pursuing origins of (poly)ethylene glycol-induced G-quadruplex structural modulations
著者
Trajkovski, Marko ; Endoh, Tamaki ; Tateishi-Karimata, Hisae ; Ohyama, Tatsuya ; Tanaka, Shigenori ; Plavec, Janez ; Sugimoto, Naoki
著者名
Trajkovski, Marko
著者名
Endoh, Tamaki
著者名
Tateishi-Karimata, Hisae
著者名
Ohyama, Tatsuya
著者ID
A0271
研究者ID
1000010379480
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=c07e328da483e766520e17560c007669
著者名
Tanaka, Shigenori
田中, 成典
タナカ, シゲノリ
所属機関名
システム情報学研究科
著者名
Plavec, Janez
著者名
Sugimoto, Naoki
言語
English (英語)
収録物名
Nucleic Acids Research
巻(号)
46(8)
ページ
4301-4315
出版者
Oxford University Press (OUP)
刊行日
2018-05-04
公開日
2018-06-06
抄録
Molecular crowding conditions provided by high concentration of cosolutes are utilized for characterization of biomolecules in cell-mimicking environment and development of drug-delivery systems. In this context, (poly)ethylene glycols are often used for studying non-canonical DNA structures termed Gquadruplexes, which came into focus by emerging structural biology findings and new therapeutic drug design approaches. Recently, several reports were made arguing against using (poly)ethylene glycols in role of molecular crowding agents due to their direct impact on DNA G-quadruplex stability and topology. However, the available data on structural details underlying DNA interaction is very scarce and thus limits in-depth comprehension. Herein, structural and thermodynamic analyses were strategically combined to assess G-quadruplex-cosolute interactions and address previously reported variances regarding the driving forces of G-rich DNA structural transformations under molecular crowding conditions. With the use of complementary (CD, NMR and UV) spectroscopic methods and model approach we characterized DNA G-quadruplex in the presence of the smallest and one of the largest typically used (poly)ethylene glycols. Dehydration effect is the key contributor to ethylene-glycol-induced increased stability of the G-quadruplex, which is in the case of the large cosolute mainly guided by the subtle direct interactions between PEG 8000 and the outer G-quartet regions.
カテゴリ
システム情報学研究科
学術雑誌論文
権利
© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.
CC license
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
関連情報
DOI
https://doi.org/10.1093/nar/gky250