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精密分子認識空間構築を実現する配向性分子インプリンティング

Oriented Immobilization-based Molecular Imprinting for Constructing Nanocavities Capable of Precise Molecular Recognition

Japanese (日本語)

Bunseki kagaku

68(2)

89-101

日本分析化学会

2019-02

2019-03-27

均一性の高い親和性と選択性をもつ分子認識材料は，高感度バイオセンサをはじめとした現在の分析科学とその産業への展開において欠かせない材料である．本稿では，この均一性の高い結合特性をもつことが期待される人工分子認識材料を創製する手法として，配向性分子インプリンティング技術について概説する．本手法は，鋳型分子を配向固定化し，制御/リビングラジカル重合によるボトムアップ型ポリマー薄膜形成技術を利用して精密な分子認識空間をもつ分子インプリントポリマー（MIPs）を得るものであり，従来法では発現できない高い親和性と選択性を示す分子認識空間の形成が可能となった．一連の研究で，低分子の生体成分や生物活性物質，また生体高分子であるがんマーカータンパク質や糖タンパク質が標的分子である場合の，それぞれに適した配向固定化方法を提案している．さらに本アプローチは，本稿でまとめた高感度バイオセンサの開発に限らず，高性能アフィニティ分離剤や薬物送達への応用が期待できることから，今後，バイオテクノロジーやナノテクノロジーなど様々な領域への波及効果が期待できる. Precise molecular recognition is required for various research and industrial fields, like sensing materials and pharmaceuticals. Oriented template immobilization-based molecular imprinting has been developed for creating artificial molecular recognition materials with high affinity and selectivity towards various target molecules, including small molecules, proteins, and glycoproteins. Molecularly imprinted polymers (MIPs) are artificial polymer-based molecular recognition materials. The molecular imprinting process involves a template polymerization approach, where the molecular complex of functional monomers and the template molecule is copolymerized by using crosslinking agents and a comonomer, followed by removal of the template molecules from the polymer matrix, yielding molecular recognition nanocavities bearing multiple interaction sites. The oriented immobilization of template molecules and precision polymer synthesis with strictly controlled polymer thickness produces precise molecular recognition cavities in MIPs. To date, the proposed oriented template immobilization-based molecular imprinting process has been successfully applied to polymeric thin-layer-based materials, affording highly sensitive and selective sensing materials. This approach can be applied to materials of various shapes, including particle-based materials, which leads to high-affinity chromatography agents and nanoparticle-based pharmaceutical materials. Therefore, the novel strategy based on molecular imprinting described herein can be applied to create various intelligent materials.

living radical polymerization

学術雑誌論文

© 2019 The Japan Society for Analytical Chemistry