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https://hdl.handle.net/20.500.14094/90005820

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メタデータID
90005820
アクセス権
open access
出版タイプ
Version of Record
タイトル
HT-SuperSAGE of the gut tissue of a Vip3Aa-resistant Heliothis virescens (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance
著者
Ayra-Pardo, Camilo ; Ochagavia, Maria E. ; Raymond, Ben ; Gulzar, Asim ; Rodriguez-Cabrera, Lianet ; Rodriguez de la Noval, Claudia ; Moran Bertot, Ivis ; Terauchi, Ryohei ; Yoshida, Kentaro ; Matsumura, Hideo ; Tellez Rodriguez, Pilar ; Hernandez Hernandez, Daily ; Borras-Hidalgo, Orlando ; Wright, Denis J.
著者名
Ayra-Pardo, Camilo
著者名
Ochagavia, Maria E.
著者名
Raymond, Ben
著者名
Gulzar, Asim
著者名
Rodriguez-Cabrera, Lianet
著者名
Rodriguez de la Noval, Claudia
著者名
Moran Bertot, Ivis
著者名
Terauchi, Ryohei
著者ID
A1340
研究者ID
1000040570750
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=5a71d33bad70874b520e17560c007669
著者名
Yoshida, Kentaro
吉田, 健太郎
ヨシダ, ケンタロウ
所属機関名
農学研究科
著者名
Matsumura, Hideo
著者名
Tellez Rodriguez, Pilar
著者名
Hernandez Hernandez, Daily
著者名
Borras-Hidalgo, Orlando
著者名
Wright, Denis J.
言語
English (英語)
収録物名
Insect Science
巻(号)
26(3)
ページ
479-498
出版者
Wiley
刊行日
2019-06
公開日
2019-04-11
抄録
Multitoxin Bt-crops expressing insecticidal toxins with different modes of action, for example, Cry and Vip, are expected to improve resistance management in target pests. While Cry1A resistance has been relatively well characterized in some insect species, this is not the case for Vip3A, for which no mechanism of resistance has yet been identified. Here we applied HT-SuperSAGE to analyze the transcriptome of the gut tissue of tobacco budworm Heliothis virescens (F.) laboratory-selected for Vip3Aa resistance. From a total of 1 324 252 sequence reads, 5 895 126-bp tags were obtained representing 17 751 nonsingleton unique transcripts (UniTags) from genetically similar Vip3Aa-resistant (Vip-Sel) and susceptible control (Vip-Unsel) strains. Differential expression was significant (≥2.5 fold or ≤0.4; P < 0.05) for 1989 sequences (11.2% of total UniTags), where 420 represented overexpressed (OE) and 1569 underexpressed (UE) genes in Vip-Sel. BLASTN searches mapped 419 UniTags to H. virescens sequence contigs, of which, 416 (106 OE and 310 UE) were unambiguously annotated to proteins in NCBI nonredundant protein databases. Gene Ontology distributed 345 of annotated UniTags in 14 functional categories with metabolism (including serine-type hydrolases) and translation/ribosome biogenesis being the most prevalent. A UniTag homologous to a particular member of the REsponse to PAThogen (REPAT) family was found among most overexpressed, while UniTags related to the putative Vip3Aa-binding ribosomal protein S2 (RpS2) were underexpressed. qRT-PCR of a subset of UniTags validated the HT-SuperSAGE data. This study is the first providing lepidopteran gut transcriptome associated with Vip3Aa resistance and a foundation for future attempts to elucidate the resistance mechanism.
キーワード
Bt resistance
Heliothis virescens
HT-SuperSAGE
transcriptomics
Vip3Aa
カテゴリ
農学研究科
学術雑誌論文
権利
© 2017 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.
CC license
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
関連情報
DOI
https://doi.org/10.1111/1744-7917.12535