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https://hdl.handle.net/20.500.14094/90007637
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2024-09-01
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90007637 (fulltext)
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1.36 MB
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メタデータID
90007637
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity
著者
Matsumura, Hiroyoshi ; Shiomi, Keita ; Yamamoto, Akito ; Taketani, Yuri ; Kobayashi, Noriyuki ; Yoshizawa, Takuya ; Tanaka, Shun-ichi ; Yoshikawa, Hiroki ; Endo, Masaki ; Fukayama, Hiroshi
著者名
Matsumura, Hiroyoshi
著者名
Shiomi, Keita
著者名
Yamamoto, Akito
著者名
Taketani, Yuri
著者名
Kobayashi, Noriyuki
著者名
Yoshizawa, Takuya
著者名
Tanaka, Shun-ichi
著者名
Yoshikawa, Hiroki
著者名
Endo, Masaki
著者ID
A1054
研究者ID
1000060373255
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=ffb2e3a5f7627a79520e17560c007669
著者名
Fukayama, Hiroshi
深山, 浩
フカヤマ, ヒロシ
所属機関名
農学研究科
言語
English (英語)
収録物名
Molecular Plant
巻(号)
13(11)
ページ
1570-1581
出版者
Elsevier (Cell Press)
刊行日
2020-11-02
公開日
2021-12-01
抄録
Photosynthetic rate at the present atmospheric condition is limited by the CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) because of its extremely low catalytic rate (kcat) and poor affinity for CO2 (Kc) and specificity for CO2 (Sc/o). Rubisco in C4 plants generally shows higher kcat than that in C3 plants. Rubisco consists of eight large subunits and eight small subunits (RbcS). Previously, the chimeric incorporation of sorghum C4-type RbcS significantly increased the kcat of Rubisco in a C3 plant, rice. In this study, we knocked out rice RbcS multigene family using the CRISPR-Cas9 technology and completely replaced rice RbcS with sorghum RbcS in rice Rubisco. Obtained hybrid Rubisco showed almost C4 plant-like catalytic properties, i.e., higher kcat, higher Kc, and lower Sc/o. Transgenic lines expressing the hybrid Rubisco accumulated reduced levels of Rubisco, whereas they showed slightly but significantly higher photosynthetic capacity and similar biomass production under high CO2 condition compared with wild-type rice. High-resolution crystal structural analysis of the wild-type Rubisco and hybrid Rubisco revealed the structural differences around the central pore of Rubisco and the βC-βD hairpin in RbcS. We propose that such differences, particularly in the βC-βD hairpin, may impact the flexibility of Rubisco catalytic site and change its catalytic properties.
キーワード
CRISPR-Cas9
C4 plant
photosynthesis
rice
Rubisco
カテゴリ
農学研究科
学術雑誌論文
権利
© The Author 2020.
This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
関連情報
DOI
https://doi.org/10.1016/j.molp.2020.08.012
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資源タイプ
journal article
ISSN
1674-2052
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eISSN
1752-9867
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NCID
AA12310973
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