90008035

open access

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FGF-23 from erythroblasts promotes hematopoietic progenitor mobilization

English (英語)

Blood

137(11)

1457-1467

American Society of Hematology

2021-03-18

2022-04-01

Fibroblast growth factor 23 (FGF-23) hormone is produced by bone-embedded osteocytes and regulates phosphate homeostasis in kidneys. We found that administration of granulocyte colony-stimulating factor (G-CSF) to mice induced a rapid, substantial increase in FGF-23 messenger RNA in bone marrow (BM) cells. This increase originated mainly from CD45−Ter119+CD71+ erythroblasts. FGF-23 protein in BM extracellular fluid was markedly increased during G-CSF–induced hematopoietic progenitor cell (HPC) mobilization, but remained stable in the blood, with no change in the phosphate level. Consistent with the BM hypoxia induced by G-CSF, low oxygen concentration induced FGF-23 release from human erythroblast HUDEP-2 cells in vitro. The efficient mobilization induced by G-CSF decreased drastically in both FGF-23−/− and chimeric mice with FGF-23 deficiency, only in hematopoietic cells, but increased in osteocyte-specific FGF-23−/− mice. This finding suggests that erythroblast-derived, but not bone-derived, FGF-23 is needed to release HPCs from BM into the circulation. Mechanistically, FGF-23 did not influence CXCL-12 binding to CXCR-4 on progenitors but interfered with their transwell migration toward CXCL-12, which was canceled by FGF receptor inhibitors. These results suggest that BM erythroblasts facilitate G-CSF–induced HPC mobilization via FGF-23 production as an intrinsic suppressor of chemoattraction.

osteocytes

学術雑誌論文

This research was originally published in Blood. Shinichi I., Tomohide S., Kanako W., Noboru A., Yuko K., Hiroki K., Akiko S., Kentaro M., Yukio N., Seiya M., Satoru T., Toshimitsu M., Yoshio K. FGF-23 from erythroblasts promotes hematopoietic progenitor mobilization. Blood. 2021;137(11):1457–1467. © 2021 by The American Society of Hematology