神戸大学附属図書館デジタルアーカイブ
https://hdl.handle.net/20.500.14094/90009263

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メタデータID
90009263
アクセス権
open access
出版タイプ
Accepted Manuscript
タイトル
Enhancement of astaxanthin incorporation by pulsed high-intensity ultrasound in LPS-stimulated macrophages
著者
Ma, Xiaoqi ; Yamaguchi, Atomu ; Maeshige, Noriaki ; Uemura, Mikiko ; Noguchi, Hikari ; Kondo, Hiroyo ; Fujino, Hidemi
著者名
Ma, Xiaoqi
著者名
Yamaguchi, Atomu
著者ID
A1349
研究者ID
1000090617838
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=bad2e50ccfd61e15520e17560c007669
著者名
Maeshige, Noriaki
前重, 伯壮
マエシゲ, ノリアキ
所属機関名
保健学研究科
著者名
Uemura, Mikiko
著者名
Noguchi, Hikari
著者名
Kondo, Hiroyo
著者ID
A1223
研究者ID
1000020278998
KUID
https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=becd953e41b2f244520e17560c007669
著者名
Fujino, Hidemi
藤野, 英己
フジノ, ヒデミ
所属機関名
保健学研究科
言語
English (英語)
収録物名
Journal of Medical Ultrasonics
巻(号)
49(2)
ページ
125-132
出版者
Springer
刊行日
2022-04
公開日
2023-02-01
抄録
Purpose Ultrasound (US) has been reported to improve the permeability of cell membranes to pharmaceuticals by causing cavitation. Astaxanthin (AX) potently terminates the induction of inflammation, but it has low oral bioavailability, which limits its incorporation in local cells and organs and its therapeutic potential. In this study, we aimed to investigate the contribution of US to AX incorporation to compensate for the limited incorporation of AX, and regulation of the pro-inflammatory factor interleukin-1β (IL-1β) by AX. Methods Murine bone marrow-derived macrophages were stimulated by lipopolysaccharide (LPS). After 2 h, cells were treated with 10 μM AX and/or pulsed high-intensity US irradiation. The cells were then incubated for another 3 h and harvested. AX incorporation in cells was measured by absorbance, and the expression of IL-1β was measured by qPCR. All values are expressed as means ± standard error of the mean. Results The combination of AX and US significantly increased AX incorporation in cells compared to AX alone (p < 0.05). In addition, this combination further suppressed the expression of IL-1β compared to AX alone (p < 0.05). Conclusion Pulsed high-intensity US irradiation combined with AX treatment promoted AX incorporation in cells and enhanced the anti-inflammatory effect on macrophages.
キーワード
Astaxanthin (AX)
Pulsed high-intensity ultrasound
Inflammation
カテゴリ
保健学研究科
学術雑誌論文
権利
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature's AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s10396-022-01189-4
関連情報
DOI
https://doi.org/10.1007/s10396-022-01189-4