c-Src-mediated phosphorylation and activation of kinesin KIF1C promotes elongation of invadopodia in cancer cells

Saji, Takeshi; Nishita, Michiru; Ikeda, Kazuho; Endo, Mitsuharu; Okada, Yasushi; Minami, Yasuhiro

https://hdl.handle.net/20.500.14094/90009458

このアイテムのアクセス数:89件（2025-04-30 12:42 集計）

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メタデータID	90009458
アクセス権	open access
出版タイプ	Version of Record
タイトル	c-Src-mediated phosphorylation and activation of kinesin KIF1C promotes elongation of invadopodia in cancer cells
著者	Saji, Takeshi ; Nishita, Michiru ; Ikeda, Kazuho ; Endo, Mitsuharu ; Okada, Yasushi ; Minami, Yasuhiro
著者名 Saji, Takeshi
著者ID A0785 研究者ID 1000030379359 著者名 Nishita, Michiru 西田, 満ニシタ, ミチル所属機関名医学研究科
著者名 Ikeda, Kazuho
著者ID A0803 研究者ID 1000090436444 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=1ae999f6941909eb520e17560c007669 著者名 Endo, Mitsuharu 遠藤, 光晴エンドウ, ミツハル所属機関名医学研究科
著者名 Okada, Yasushi
著者ID A0499 研究者ID 1000070229772 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=9f86495b89d5a974520e17560c007669 著者名 Minami, Yasuhiro 南, 康博ミナミ, ヤスヒロ所属機関名医学研究科
言語	English (英語)
収録物名	Journal of Biological Chemistry
巻(号)	298(7)
ページ	102090
出版者	Elsevier
刊行日	2022-07
公開日	2022-07-08
抄録	Invadopodia on cancer cells play crucial roles in tumor invasion and metastasis by degrading and remodeling the surrounding extracellular matrices and driving cell migration in complex 3D environments. Previous studies have indicated that microtubules (MTs) play a crucial role in elongation of invadopodia, but not their formation, probably by regulating delivery of membrane and secretory proteins within invadopodia. However, the identity of the responsible MT-based molecular motors and their regulation has been elusive. Here, we show that KIF1C, a member of kinesin-3 family, is localized to the tips of invadopodia and is required for their elongation and the invasion of cancer cells. We also found that c-Src phosphorylates tyrosine residues within the stalk domain of KIF1C, thereby enhancing its association with tyrosine phosphatase PTPD1, that in turn activates MT-binding ability of KIF1C, probably by relieving the autoinhibitory interaction between its motor and stalk domains. These findings shed new insights into how c-Src signaling is coupled to the MT-dependent dynamic nature of invadopodia and also advance our understanding of the mechanism of KIF1C activation through release of its autoinhibition.
キーワード	cell invasion
	invadopodia
	KIF1C
	kinesin
	microtubule
	phosphorylation
	Src
	tumor cell biology
カテゴリ	医学研究科
カテゴリ	学術雑誌論文
権利	© 2022 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.
権利	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
関連情報	DOI https://doi.org/10.1016/j.jbc.2022.102090

資源タイプ	journal article
ISSN	0021-9258　OPACで所蔵を検索　 CiNiiで学外所蔵を検索

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