E0034102

open access

Version of Record

Suppressive effect of 1,25(OH)2D3, and glucocorticoids on production of tumor necrosis factor-alpha by human peripheral blood adherent cells.

English (英語)

The Kobe journal of the medical sciences

37(3)

179-188

1991

Effect of 1,25(OH)2D3 and glucocorticoids on production of TNF-alpha by human peripheral blood adherent cells (HPBAC) was investigated. TNF-alpha was measured by ELISA method using monoclonal antibody against human recombinant THF-alpha. Maximal TNF-alpha production by HPBAC was observed in 4 to 8 hr after incubation when these cells were cultured for 24 hr with 10 micrograms/ml lipopolysaccharide (LPS), 10 ng/ml 12-o-tetradecanoyl-phorbol-13-acetate (TPA) and 10(-5) M indomethacin (INDM). When graded concentration of 1,25(OH)2D3 was added to HPBAC in the presence of above stated dose of LPS, TPA and INDM, a significant suppression of TNF-alpha production was first observed at the concentration of 10(-8) M (P less than 0.05). Similar significant suppressive effect was also observed by adding hydrocortisone and dexamethasone at the concentration of 10(-8) M (P less than 0.05) and 10(-7) M (P less than 0.05), respectively. These results may suggest that 1,25(OH)2D3 has the same inhibitory effect on monocyte/macrophage function as glucocorticoids.

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